A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS)

Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, necessity activate with water, and short duration motility. To achieve standardization sperm activation, microfluidic mixers have shown improved reproducibility over activation by hand, but challenges remain in optimizing simplifying use these microdevices for greater adoption. The device described herein incorporates a novel micromixer geometry that aligns two inlet streams modified herringbone structures split recombine at 1:6 dilution water rapid consistent initiation polydimethylsiloxane (PDMS) chip can be operated positive or negative pressure configuration, allowing simple micropipettor draw samples into rapidly stop flow. was optimized not only zebrafish also enables practical standard computer-assisted (CASA) systems. could other differing cell sizes adopted an open hardware approach using 3D resin printing where users revise, fabricate, share designs improve across laboratories repositories.